An electron microscope can only operate inside a vacuum chamber. A good vacuum, needed so that electrons do not collide with air molecules or dirt before reaching the sample and the detector coming back, would be 1e-7 Torr or below. Your sample (the object of interest) has to be small enough to be mounted on a pedestal, which is inserted into chamber where the electron beam will be. The pedestal has to have multiple degrees of freedom so the e-beam can interrogate at various angle and position. The last requirement would be that your sample does not charge up too easily, which will distort the image. Charging can be lessened with a flash of gold.
Hence, if your sample meets the requirements listed, you should be able to see its image on a monitor just as clearly as you see an object of a much larger scale, with an optical microscope. Any items that are detrimental to a vacuum formation (moist, spongy materials, for example) should probably be avoided. From an electron microscope, I have seen something as big as the compound eyes of a fly or a feature as small as 1 nm.
Under a transmission electron microscope, one can see the internal structure of cells, tissues, and individual molecules at a very high magnification. This type of microscope can reveal details such as the arrangement of atoms and the ultrastructure of organelles within cells.
Individual cells are not visible under an electron microscope because the resolution of the microscope is not high enough to distinguish the borders of individual cells. The electron microscope's resolution is limited by the wavelength of electrons used, preventing the visualization of single cells. Cells can be seen collectively as tissues or structures under an electron microscope.
The minimum size of an organism that can be seen under a light microscope is around 0.2 micrometers, which is the limit of resolution for this type of microscope. Anything smaller than that would require an electron microscope for visualization.
An electron microscope, particularly a transmission electron microscope (TEM), allows you to see inside the cell and view organelles in detail. It provides high magnification and resolution to observe the internal structures of cells. However, bacteria can also be visualized using a light microscope or a scanning electron microscope (SEM).
You would typically need an electron microscope to see the details inside a bacterium, as the resolution of an electron microscope is much higher than that of a light microscope. Electron microscopes use a beam of electrons to create an image, allowing for detailed visualization of the internal structures of bacteria.
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Under a transmission electron microscope, one can see the internal structure of cells, tissues, and individual molecules at a very high magnification. This type of microscope can reveal details such as the arrangement of atoms and the ultrastructure of organelles within cells.
Individual cells are not visible under an electron microscope because the resolution of the microscope is not high enough to distinguish the borders of individual cells. The electron microscope's resolution is limited by the wavelength of electrons used, preventing the visualization of single cells. Cells can be seen collectively as tissues or structures under an electron microscope.
Under an electron microscope, structures can be seen with much higher resolution compared to a light microscope. This includes viewing individual molecules, detailed cell structures, and even viruses. The high magnification and resolution of an electron microscope allow for visualization of fine details that are not visible under a light microscope.
An electron microscope would be needed to see detailed images of an internal structure of a bacterium, as it can provide much higher magnification and resolution compared to a light microscope. The electron microscope uses a beam of electrons instead of light to visualize the specimen, allowing for better visualization of internal structures at the cellular level.
Subcellular structures like ribosomes, protein complexes, and viruses can be seen under an electron microscope due to its higher magnification and resolution capabilities compared to a light microscope. The electron microscope uses a beam of electrons to create an image, providing much finer details than a light microscope that uses visible light.
electron tunneling microscope
A Transmission Electron Microscope
The compound microscope is a light microscope that uses light to "see" microbes. Viruses are too small.The electron microscope uses electrons to "see" microbes or viruses.
A single atom is not visible in a microscope (it is too small to be imaged by photons). What you see in an optical microscope (or in general) is the light reflected, scattered, or emitted by the electron layers of the material under observation.
Electron microscope