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seeding density of the cell

quality of transfecting dna

ratio of dna to transfectant reagent

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13y ago
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4mo ago

Factors that can affect transfection efficiency include the type of transfection reagent used, the concentration of DNA or RNA being transfected, the cell type being transfected, and the health and confluency of the cells. The choice of transfection method, such as chemical or viral transfection, can also impact efficiency. The presence of serum or antibiotics in the culture medium, as well as the timing of transfection, can also influence transfection efficiency.

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Q: What are the factors that can affect the transfection efficiency and how?
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