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SDS lyses the cells. Tris controls the pH. Glucose prepares bacterial DNA. EDTA protects DNA from degradation. Phenol extracts lipids and proteins from DNA. Chilled absolute ethanol precipitates the DNA.

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βˆ™ 10y ago
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βˆ™ 5mo ago
  1. SDS (sodium dodecyl sulfate): acts as a detergent to break down cell membranes and nuclear envelopes, releasing DNA.
  2. Proteinase K: digests proteins (like histones) that bind to DNA, allowing DNA molecules to be released.
  3. Ethanol: used to precipitate DNA out of solution by breaking hydrophilic interactions between DNA and water, facilitating DNA isolation.
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Q: Specific functions of each chemicals used in DNA extraction?
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