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Since you know what Glucose Oxidase is I'm assuming you know what enzymes are. If not, an enzyme is a protein that catalyzes chemical reactions.
Glucose oxidase is the enzyme built specifically for Glucose. Glucose Oxidase binds to the six-carbon sugar Glucose and aids the organism in breaking it down into metabolites.
Glucose reacts with glucose oxidase by binding to the enzyme's active site. Glucose oxidase then catalyzes the oxidation of glucose to produce gluconic acid and hydrogen peroxide. This reaction can be used to detect or quantify glucose levels in various samples.
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Why is the Glucose Oxidase test a more specific test for urinary glucose that Benedict's test?
The Glucose Oxidase test specifically measures the presence of glucose by detecting its oxidation reaction with glucose oxidase enzyme. This enzyme only reacts with glucose, making the test highly specific for glucose detection. On the other hand, Benedict's test, which relies on the reduction of copper ions, can give false positive results with other reducing sugars present in the urine, leading to lower specificity for glucose.
Is Pseudomonas luteola oxidase positive?
Yes, Pseudomonas luteola is oxidase-positive, meaning it contains the enzyme cytochrome C oxidase which catalyzes the oxidation of cytochrome C. This can be detected in the laboratory using an oxidase test.
What is the formula that use to calculate the concentration of glucose in blood by the principle of Beer-Lambert law and application of glucose oxidase?
To calculate the concentration of glucose in blood using the Beer-Lambert law principle and glucose oxidase, you would typically measure the absorbance of a glucose solution with a spectrophotometer at a specific wavelength. The formula to calculate the concentration of glucose is: Glucose concentration (mg/dL) = (Absorbance - intercept) / slope Where the slope and intercept are obtained from a calibration curve using known concentrations of glucose.
What happens when glucose and starch react together?
Glucose and starch do not react together chemically. However, enzymes such as amylase can break down starch into glucose through a process called hydrolysis. This allows the glucose to be used for energy production in the body.
How can you easily distinguish enterics and pseudomonads?
Enterics are typically Gram-negative bacteria that ferment glucose, produce acid, and are often found in the intestines of animals. Pseudomonads are also Gram-negative bacteria, but they do not ferment glucose. Additionally, pseudomonads are known for their ability to produce pyocyanin pigment and grow in diverse environments, such as soil and water.
What is the molecular weight of glucose oxidase?
The molecular weight of glucose oxidase is approximately 160-190 kDa, depending on the specific source and form of the enzyme.
How do clinistix work?
Clinistix are used to test for glucose in urine by detecting the presence of reducing sugars. When dipped in urine, the strip will change color based on the amount of glucose present. This color change is then matched to a color chart to determine the glucose level in the urine sample.
Why does catalase elute in a wider range of fractions than glucose oxidase does?
Catalase may elute in a wider range of fractions than glucose oxidase due to differences in their molecular weights, hydrophobicity, and interactions with the gel filtration or chromatography resin. Catalase is a larger and more complex protein compared to glucose oxidase, which can lead to a broader elution profile. Additionally, catalase may have different binding affinities or interactions with the resin, resulting in varied elution behavior.
Which is the enzyme which catalyses the reaction of glucose to gluconic acid?
Glucose oxidase that converts the carbonyl (aldehyde) carbon of glucose to a carboxylic acid.
Why is the Glucose Oxidase test a more specific test for urinary glucose that Benedict's test?
The Glucose Oxidase test specifically measures the presence of glucose by detecting its oxidation reaction with glucose oxidase enzyme. This enzyme only reacts with glucose, making the test highly specific for glucose detection. On the other hand, Benedict's test, which relies on the reduction of copper ions, can give false positive results with other reducing sugars present in the urine, leading to lower specificity for glucose.
What of these substances is an enzyme A. triglyceride B. glucose C. oxidase D. omega-3 fatty acid E. nucleotide?
C. Oxidase is an enzyme.
Is Pseudomonas luteola oxidase positive?
Yes, Pseudomonas luteola is oxidase-positive, meaning it contains the enzyme cytochrome C oxidase which catalyzes the oxidation of cytochrome C. This can be detected in the laboratory using an oxidase test.
What is glucose oxidase peroxidase aminophenazone phenol?
Its a reagent used to determine the glucose content of a sample. its based on 2 coupled enzyme reactions with a colorimetric end-point: D-glucose + O2 + H2O ----- glucose oxidase ------> H2O2 + gluconate aminophenazone + phenol + H2O2 -----peroxidase----> a red dye + H2O (NOTE: H2O2 product of 1st reaction acts as substrate for 2nd) this red coloured sample can then be put into a spectrophotometer and an absorbency reading can be taken, this reading can be compared to a calibration curve and the content of glucose can be ascertained.
When glucose is burned which gas in the air does the glucose react with?
It is oxygen.
How much quantity of glucose oxidase for 1 IU?
The quantity of glucose oxidase for 1 IU can vary depending on the specific enzyme preparation and assay method used. Generally, 1 IU of glucose oxidase is defined as the amount of enzyme that catalyzes the oxidation of 1 micromole of glucose per minute under specific conditions. It is typically around 1 microgram of enzyme, but it is important to refer to the manufacturer's instructions for the specific enzyme preparation being used.
What is the formula that use to calculate the concentration of glucose in blood by the principle of Beer-Lambert law and application of glucose oxidase?
To calculate the concentration of glucose in blood using the Beer-Lambert law principle and glucose oxidase, you would typically measure the absorbance of a glucose solution with a spectrophotometer at a specific wavelength. The formula to calculate the concentration of glucose is: Glucose concentration (mg/dL) = (Absorbance - intercept) / slope Where the slope and intercept are obtained from a calibration curve using known concentrations of glucose.
What chemical detects glucose?
A chemical called glucose oxidase is commonly used to detect glucose. This enzyme reacts with glucose in the presence of oxygen, producing hydrogen peroxide as a byproduct. The level of hydrogen peroxide produced is then typically measured as an indicator of the glucose concentration in a sample.
