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Data Interpretation for an MMT assay: Absorbance (OD) values that are lower than the control cells indicate a reduction in the rate of cell proliferation. Conversely a higher absorbance rate indicates an increase in cell proliferation. Rarely, an increase in proliferation may be offset by cell death; evidence of cell death may be inferred from morphological changes.
The O.D values from an MTT assay are used to measure metabolic activity of cells, not cell count. Higher O.D values indicate higher metabolic activity in the cells, which can be related to cell viability or proliferation. To relate O.D values to cell count, one would need to establish a standard curve correlating O.D values to known cell counts.
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If the mitochondrion is fundmental to the mtt assay which organelle plays a key role in the neutral red viability assay and why?
The lysosome plays a key role in the neutral red viability assay. Neutral red is taken up by lysosomes and accumulates in these acidic organelles in viable cells, producing a red color. This assay measures lysosomal function as an indicator of cell viability and health.
Does the number of bacteria recorded by the standard plate count method accurately reflect the total bacterial count from the sample?
No, the standard plate count method only measures viable bacteria that can grow under the specific conditions used in the assay. It does not account for non-viable bacteria or those that may not grow under the conditions provided, so it may not provide an accurate reflection of the total bacterial count in the sample.
What does blood test MID measure?
Blood test MID measures the percentage of mixed cell populations, including monocytes, eosinophils, and basophils, in the white blood cell count. MID values can help identify and monitor certain health conditions like infections, inflammation, and autoimmune diseases.
Can eczema cause low white blood cell count?
Eczema is a skin condition and is not typically associated with causing a low white blood cell count. Low white blood cell count can be due to other underlying conditions such as infections, certain medications, autoimmune disorders, or bone marrow disorders. It is important to consult a healthcare provider for proper evaluation and diagnosis.
What does sfemlin look for in a blood test?
Sfemlin typically looks for red blood cell count, white blood cell count, platelet count, hemoglobin levels, and various other blood components that can indicate overall health and potential medical conditions.
What is the syntax for a COUNT function?
=COUNT(cells) Cells can be the reference to the cell range you want to count. Say you want to count values in the cells from A2 to V20, then you would do it this way: =COUNT(A2:V20)
How to read the results of hematology?
To read the results of a hematology test, look at values such as red blood cell count, white blood cell count, hemoglobin, hematocrit, and platelet count. Compare these values to the reference ranges provided by the laboratory. Values within the reference range are considered normal, while values outside the range may indicate an underlying medical condition. If you have concerns about your results, consult with a healthcare provider for further interpretation and guidance.
How do you diagnose CMV?
There are various ways in which to diagnose CMV (cytomegalovirus): 1. a cell culture from the urine, pharynx, or blood 2. cell recovery from an organ 3. an antibody increase in the blood 4. a PCR (polymerase chain reaction) assay 5. an immunofluorescent assay 6. an indirect hemagglutination assay and 7. a latex agglutination assay.
Are there critical or panic values for a red blood cell count?
There is not a critical/panic value associated with red blood cell counts. The RBC is usually not clinically significant but the hemoglobin and hematocrit are. Hematocrit is calculated using the red count, that is why it is needed.
What would the cell wall relate to in a car?
The cell wall of a cell would relate to the body/exterior of the car
What is a competition assay?
A completion assay is an experiment in which the cell growth and death of two cell lines, one with the gene of interest silenced and the other used as a control, are compared to draw conclusions about the effect of knocking down the gene of interest.
What is the ELISA-ACT short for?
Enzyme-linked immunoserological assay activated cell test
What is clonogenic assay?
A clonogenic assay is a laboratory technique used to measure the survival and growth potential of individual cells following treatment with drugs, radiation, or other stress-inducing factors. In this assay, cells are seeded at low densities to allow for the formation of colonies derived from a single progenitor cell, which are then stained and counted to assess cell viability and reproductive capacity. The results of the clonogenic assay provide valuable information about the effectiveness of treatments in inhibiting cell proliferation and inducing cell death.
What is the full name of MTT assay that is used to measure the cell activity?
The full name of MTT assay is 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide assay. It is a colorimetric assay commonly used to measure cell viability and proliferation by assessing the metabolic activity of cells, mainly through the activity of mitochondrial dehydrogenase enzymes.
What is the difference between count and countA?
Count: counts the number of cells with numeric values in a range. Counta: counts the number of cells containing data (letters or numbers) in a range. Counta counts the number of non-blank cells in a range. Counta will therefore exclude any empty cells, which would be included if the count function were used.
How to choose using an MTT assay vs Crystal Violet Staining?
MTT assay is used to measure cell viability by assessing metabolic activity, while crystal violet staining is used to quantify cell proliferation by staining total cellular biomass in a population. Choose MTT assay when you want to measure cell viability specifically, and crystal violet staining when you want to evaluate overall cell proliferation in a population. Each method provides different information about cell health and growth.
What are some lab terms?
WBC = white bloodcell count RBC = red blood cell count MCV = mean cell volume MCH = mean cell hemoglobine RDW = random distribution width (doesn't say that much in a CBC) Ab lymp = lymphocytes absolute (= percentage of lymphocytes X wbc) Please take a closer look at the lab report, the reference values (= values of a healthy subject) should be on there too.
