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The CRISPR-Cas9 system induces double strand breaks in DNA by using a guide RNA to target a specific sequence in the DNA. The Cas9 enzyme then cuts the DNA at that location. The cell's repair machinery can then fix the break by either joining the broken ends back together or by introducing new genetic material.

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Q: How does the CRISPR-Cas9 system induce and repair double strand breaks in DNA?
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How does the cell repair a double strand break in DNA?

When a cell encounters a double strand break in DNA, it activates a repair process called homologous recombination. In this process, the cell uses a matching DNA sequence from a sister chromatid as a template to repair the broken strands. Enzymes help align the broken DNA ends with the template, allowing for accurate repair of the double strand break.


How does DNA ligase utilize ATP during the process of DNA repair and replication?

DNA ligase utilizes ATP during the process of DNA repair and replication by using the energy from ATP to catalyze the formation of a phosphodiester bond between the DNA strands, sealing any breaks or gaps in the DNA molecule.


Why are checkpoints important to the health of the cells?

Checkpoints are important in cell cycle regulation as they ensure that each phase of the cell cycle is completed accurately before progressing to the next phase. Checkpoints help to prevent the proliferation of damaged or mutated cells, thus maintaining cellular health and preventing the development of diseases like cancer. They enable cells to repair DNA damage or induce apoptosis if the damage is too severe for repair.


When a bone breaks what carry the food into the bone?

Blood vessels supply the bone with nutrients and carry food and oxygen to the bone cells. When a bone breaks, blood vessels at the site of the fracture help in the healing process by delivering the necessary nutrients for bone repair.


What is Nucleotide excision repair mechanism?

Nucleotide excision repair is a DNA repair mechanism that removes bulky DNA lesions, such as UV-induced thymine dimers or chemical adducts, by cutting out a patch of nucleotides surrounding the damaged site and replacing it with newly synthesized DNA. This process involves multiple proteins working together to recognize, excise, and repair the damaged DNA. It is crucial for maintaining genomic stability and preventing mutations that can lead to diseases like cancer.

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