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The CRISPR-Cas9 system induces double strand breaks in DNA by using a guide RNA to target a specific sequence in the DNA. The Cas9 enzyme then cuts the DNA at that location. The cell's repair machinery can then fix the break by either joining the broken ends back together or by introducing new genetic material.

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Q: How does the CRISPR-Cas9 system induce and repair double strand breaks in DNA?
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How does the cell repair a double strand break in DNA?

When a cell encounters a double strand break in DNA, it activates a repair process called homologous recombination. In this process, the cell uses a matching DNA sequence from a sister chromatid as a template to repair the broken strands. Enzymes help align the broken DNA ends with the template, allowing for accurate repair of the double strand break.


What are the key differences between non-homologous end joining and homology-directed repair in DNA repair mechanisms?

Non-homologous end joining is a DNA repair mechanism that directly joins broken DNA ends without requiring a template, leading to potential errors. Homology-directed repair, on the other hand, uses a template from a sister chromatid to accurately repair DNA breaks, reducing the risk of errors.


How does DNA ligase utilize ATP during the process of DNA repair and replication?

DNA ligase utilizes ATP during the process of DNA repair and replication by using the energy from ATP to catalyze the formation of a phosphodiester bond between the DNA strands, sealing any breaks or gaps in the DNA molecule.


What is the function of DNA ligase in the process of DNA replication and repair?

DNA ligase is an enzyme that plays a crucial role in the process of DNA replication and repair by joining together the ends of DNA fragments. It helps to seal any breaks or gaps in the DNA strand, ensuring that the genetic information is accurately copied and maintained.


What is the mechanism of DNA ligase in the process of DNA replication and repair?

DNA ligase is an enzyme that plays a crucial role in the process of DNA replication and repair by joining together the ends of DNA strands. It forms a bond between the sugar-phosphate backbone of adjacent DNA fragments, sealing any breaks or gaps in the DNA molecule. This helps to ensure the integrity and stability of the DNA molecule during replication and repair processes.

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