Mycobacterium smegmatis is a rod-shaped bacterium with a length of about 2-3 micrometers and a width of around 0.5 micrometers.
Gram stain
Gram staining is a differential stain used to classify bacteria into two groups based on their cell wall composition. The basic steps include staining with crystal violet, iodine, alcohol decolorization, and counterstaining with safranin. Gram-positive bacteria retain the crystal violet stain and appear purple, while gram-negative bacteria lose the stain and appear pink/red.
Yogurt contains mostly lactic acid bacteria, which are Gram-positive bacteria. This means they will stain purple under a Gram stain.
In the flagella stain, all cells appear purple due to the basic dyes used to stain the flagella. This uniform coloration helps visualize the flagella structure under the microscope. In contrast, the Gram stain uses a series of dyes to differentiate between Gram-positive (purple) and Gram-negative (pink) cells based on their cell wall composition.
gram negetive coccus
No, Gram stain and Ziehl-Neelsen stain are two different types of staining methods used in microbiology. Gram stain is used to differentiate bacteria based on their cell wall composition (Gram-positive and Gram-negative), while Ziehl-Neelsen stain is used to detect acid-fast bacteria like Mycobacterium tuberculosis.
The color common to both the gram stain and the acid-fast stain is red/pink. In the gram stain, Gram-negative bacteria appear red or pink after staining with safranin, while in the acid-fast stain, acid-fast bacteria such as Mycobacterium species retain the red/pink color of carbol fuchsin despite decolorization with acid-alcohol.
A flourochrome stain is an ultraviolet reactive dye that shines when exposed to UV. By using this stain, acid-fast bacilli (such as mycobacterium) can be easily seen. Flourochrome is used because standard techniques such as Gram stains can result in inconclusive results.
No, the presence of gram-negative cocci in sputum gram stain does not necessarily indicate tuberculosis. Tuberculosis is caused by the bacterium Mycobacterium tuberculosis, which is acid-fast and appears pink on a Ziehl-Neelsen stain. Gram-negative cocci are more commonly associated with other bacterial infections, such as Haemophilus influenzae or Moraxella catarrhalis.
Examples of acid-fast organisms include Mycobacterium tuberculosis, Mycobacterium leprae, and Nocardia species. Acid-fast staining is a technique used to detect these bacteria, as they have a waxy substance in their cell walls that makes them resistant to standard staining methods.
Mycobacterium smegmatis is a rod-shaped bacterium with a length of about 2-3 micrometers and a width of around 0.5 micrometers.
Both stain poorly with the Gram stain.However Mycoplasma has no cell wall while Mycobacterium has a thick cell wall of mycolic acids.
Gram-negative on a Serratia marcescens gram stain means that the bacterium has a cell wall that does not retain the crystal violet stain used in the gram staining procedure. This indicates that Serratia marcescens has a thin peptidoglycan cell wall, and the outer membrane contains lipopolysaccharides.
It is purple. The bacteria that cause it are gram-positive, which means that under the gram stain they become purple. Your lungs produce the lung stain when they are sick, so pneumonia is purple.
Gram stain
No, TB (Mycobacterium tuberculosis) is a gram-positive bacteria.