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Bacterial growth can be measured by different ways: The most obvious way to determine microbial numbers is through direct counting. Using counting chamber like PETROFF-HAUSSER counting chamber and HEMOCYTOMETER ( it is also used for eukaryotic cells). The recent counting technique is flow cytometer, in this bacterial suspension is forced through the a small hole or orifice in the Coulter counter chamber and an electric field is applied through the hole and electrodes are placed on both sides of the orifice every time the microbial cell pass will cut the potential and electrical resistance will be noted, which give the number of cells. Onether technique is the membrane filter technique in which the sample is first filtered through a black polycarbonate membrane filter. Then bacteria are stained with a flouroscent dye such as acridine orange, and observed microscopically. The stained cells are easily observed against the black background of the membrane filter and can be counted when viewed with an epifluorescense microscope. Bacterial growth can also be measured by colony counting method.
Bacterial growth can be measured by counting the number of colonies on a plate using a colony counter, measuring turbidity with a spectrophotometer, or counting cells under a microscope using a hemocytometer. Alternatively, bioluminescent reporter genes can be used to measure growth based on light emission.
A direct chemical method for measuring cell growth is by measuring a chemical component of the cell such as total protein or total DNA content. An indirect measurement measures chemical activity such as the rate of oxygen consumption.
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Discuss a bacterial growth curve and phases of bacterial growth?
A bacterial growth curve demonstrates the pattern of bacterial population growth over time. The curve typically includes lag phase (initial period of adjustment), exponential phase (rapid growth), stationary phase (growth plateaus as resources deplete), and death phase (population decline). Understanding these phases is crucial in studying microbiology, as they provide insights into how bacteria respond to environmental conditions.
What did the control broth inoculated with Escherichia coli demonstrate?
The control broth inoculated with Escherichia coli should demonstrate bacterial growth as expected. If there is no growth in the control broth, it may indicate issues with the inoculation process, the broth itself, or other experimental variables affecting bacterial growth.
Does Bacterial growth affect temperature?
Yes. Bacterial growth increases with temperature or humidity. Lower temperatures will inhibit or completely stop bacterial growth, but not kill bacteria. The best temperature for fastest growth of some bacterial strains (such as E. coli) is the human body temperature at 37°C. However, most species cannot survive in extreme temperatures. One way to kill common bacterial pathogens using heat is by boiling, a common sterilization technique.
Why use isopropyl alcohol to inhibit bacterial growth?
Isopropyl alcohol is commonly used as a disinfectant because it can kill a broad range of bacteria by denaturing their proteins and dissolving their lipid membrane. It evaporates quickly, which helps in reducing bacterial contamination on surfaces. This makes it an effective choice for inhibiting bacterial growth in various applications.
Why might it be prefered to incubate medium at 37 degrees C with inoculated control then transfer tubes to refrigeator?
Incubating at 37°C promotes optimal bacterial growth for the control. Transferring to the refrigerator slows down bacterial growth and prevents overgrowth. This process allows for controlled bacterial growth and can help in analyzing the effects of the bacteria under specific conditions.
Why do you measure bacterial growth at 600nm only?
Measuring bacterial growth at 600nm is a commonly used method because it corresponds well with bacterial cell density and is a quick and convenient way to monitor growth in real-time. The wavelength of 600nm falls within the range where bacterial cells absorb light due to their pigments like chlorophyll, carotenoids, and cytochromes, making it a suitable indicator of cell growth.
What is the difference between bacterial and anti bacterial hand wash?
Bacterial hand wash favors the growth of bacteria on the surface of the skin ( hands). On the other hand ( no pun intended) ANTI bacterial hand wash does not favor bacterial growth and in fact contains agents to prevent any bacterial growth on the hands.
How is bacteria growth expressed?
Bacteria growth is commonly expressed as the increase in the number of bacteria present over time. This can be quantified by measuring the colony-forming units (CFU) or by using optical density measurements to estimate bacterial density. Growth can also be expressed as generation time, which is the time it takes for a bacterial population to double in number.
Can bacterial growth in urine result in false positive for methamphetamine?
No, drug tests are designed with things such as bacterial growth in mind.
How do you destroy bacterial growth?
by distorting it
What type of chemical agent retards bacterial growth?
Antibiotics are chemical agents that retard bacterial growth by targeting specific components of bacterial cells, which can inhibit their growth or kill them. Examples include penicillin, tetracycline, and ciprofloxacin.
How can the growth of microorganism measured?
1_A spectrophotometer is used to determine turbidity by measuring the amount of light that passes through a suspension of cells. 2_An indirect way of estimating bacterial numbers is measuring the metabolic activity of the population (for example, acid production or oxygen consumption). 3_For filamentous organisms such as fungi, measuring dry weight is a convenient method of growth measurement.
How does oxygen slow bacterial growth?
Oxygen slows bacterial growth by affecting the metabolic pathways within the bacterial cells. Aerobic bacteria require oxygen to produce energy through respiration, and when oxygen is limited, their growth is inhibited. Oxygen can also generate reactive oxygen species that damage cellular components, further inhibiting bacterial growth.
Discuss a bacterial growth curve and phases of bacterial growth?
A bacterial growth curve demonstrates the pattern of bacterial population growth over time. The curve typically includes lag phase (initial period of adjustment), exponential phase (rapid growth), stationary phase (growth plateaus as resources deplete), and death phase (population decline). Understanding these phases is crucial in studying microbiology, as they provide insights into how bacteria respond to environmental conditions.
What is the function of pyrogens?
inhibit bacterial growth
How the sound affect the bacterial growth?
pooop
What enzyme inhibits bacterial growth?
polymerase
