The Krebs cycle takes place in the mitochondria of eukaryotic cells. It is a series of chemical reactions that occur in the matrix of the mitochondria.
The proteins of the electron transport chain (ETC) are located in the inner mitochondrial membrane. This is where the series of complexes involved in electron transfer and ATP production are situated.
The 4 main stages of cellular respiration are glycolysis (in the cytoplasm), pyruvate oxidation (in the mitochondria), the citric acid cycle or Krebs cycle (in the mitochondria), and oxidative phosphorylation (in the inner mitochondrial membrane).
Yes, when glucose is catabolized under aerobic conditions, it is converted to pyruvate through glycolysis in the cytoplasm. Pyruvate then enters the mitochondria where it is converted to acetyl-CoA, which enters the Krebs cycle to produce energy in the form of ATP through oxidative phosphorylation.
The inner mitochondrial membrane utilizes a high proportion of cardiolipin, a unique phospholipid, which helps maintain membrane fluidity without the need for cholesterol. Cardiolipin is able to stabilize the structure of the membrane and regulate its fluidity without the presence of cholesterol.
Pyruvate requires the enzyme pyruvate translocase and a mitochondrial membrane potential to enter the mitochondria where it can undergo further processing in the citric acid cycle.
ATP, being a large hydrophilic molecule, cannot freely cross the inner membrane of mitochondria. It requires specific transporters, such as the adenine nucleotide translocase, for its entry into the mitochondrial matrix.
Pyruvic acid is transported into the mitochondria through a carrier protein known as the mitochondrial pyruvate carrier (MPC). The MPC uses the energy stored in the proton gradient across the mitochondrial membrane to move pyruvate against its concentration gradient. This process helps maintain the flow of pyruvate from the cytoplasm into the mitochondria for further energy production through aerobic respiration.
Pyruvate oxidation takes place in the mitochondrial matrix. Here, pyruvate is converted into acetyl-CoA by the pyruvate dehydrogenase complex, which is a critical step in aerobic respiration.
pyruvate is converted into acetyl coA in the mitochondrial matrix
No, pyruvate oxidation does not occur in the cytoplasm. It takes place in the mitochondria. Pyruvate produced in the cytoplasm during glycolysis is transported into the mitochondria for oxidation to acetyl-CoA.
Glycolysis occurs in the cytoplasm, followed by pyruvate entering the mitochondria for the TCA cycle in the mitochondrial matrix. Electron transport chain and oxidative phosphorylation take place in the inner mitochondrial membrane space.
Protons cross the inner mitochondrial membrane during ATP synthesis in a process known as oxidative phosphorylation. This movement of protons creates a gradient that is used to drive the production of ATP by ATP synthase.
Adenosine triphosphate (ATP) crosses the mitochondrial membrane to provide energy for cellular processes.
Cristae
The Krebs cycle takes place in the mitochondria of eukaryotic cells. It is a series of chemical reactions that occur in the matrix of the mitochondria.
The ribbon-like folds on the inner lining of the mitochondrial membrane are called cristae.