Applications of chromosome walking and jumping?

Answer 1

You want to know the sequence of a gene and only know the sequence of 2 DNA markers near it such as an RFLP (stands for Restriction fragment length polymorph) or another gene. Using different restriction enzymes, cut the genome of the organism to create DNA clones which overlap. For example, if the genome is ABCDEFGHI, enzyme 1 cuts it into 2 pieces to make ABCD EFGHI and enzyme 2 cuts it into 3 pieces to make ABC DEF GHI.. These pieces will over lap in sequence because the enzymes have different restriction sites.

Using the known sequence of the RFLP, a radio labeled nucleic acid probe can be made to search the genomic library. The probe will anneal to clones with overlaps, and the ends of these clones can be made into new probes to search for the adjacent sequence. This is repeated until the desired segment has been cloned. each clone is sequenced and the overlaps are compared to determine the order of the sequences.

This technique can be used to analysis diseases, such as cystic fibrosis, to look for mutations.

Answer 2

Chromosome walking is used to identify nearby genes by systematically moving along a chromosome in small steps and analyzing the genes in each step. It is useful for gene mapping and identifying the location of a specific gene. Chromosome jumping involves transferring a piece of DNA from one chromosome location to another, allowing researchers to study large regions of DNA or move genes between chromosomes for genetic engineering purposes. It is commonly used in constructing physical maps of chromosomes and studying genetic linkage.