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Usuallu 0.1% mercuric chloride is used for surface sterilization. Mercuric chloride reacs with ligth and will loose its activity if it is exposed to light, especially for a longer duration.. So usually fresh mercuric chloride is used (can be stored for a max of 1 or 2 weeks) and dont forget to store in amber coloured bottles, or cover the bottle

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Q: Why fresh mercuric chloride for tissue culture?
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