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a. Cannot kill endospores

b. Cannot inactivate viruses

c. Requires a long time to achieve sterilization

d. Cannot be used with heat-labile materials

e. Cannot be used with glassware

...must be one of these

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14y ago
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AnswerBot

7mo ago

One limitation of using an autoclave is that certain materials, such as plastics or oils, may not be able to withstand the high pressure and temperature inside the autoclave, leading to potential damage or failure. Additionally, autoclaves are not suitable for heat-sensitive materials that may degrade under the intense heat and pressure.

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10y ago

The autoclave has some disadvantages like the heat destroying plastic wear and turning pointy objects dull. Oily substances also does not work with an autoclave machine.

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Q: What is the limitation of using the autoclave?
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How can I ensure the purity of distilled water when using an autoclave for sterilization processes?

To ensure the purity of distilled water when using an autoclave for sterilization, it is important to regularly check and maintain the autoclave equipment to prevent contamination. Additionally, using high-quality distilled water and following proper sterilization procedures can help maintain the purity of the water during the sterilization process.


How does an autoclave effectively kill microorganisms?

An autoclave effectively kills microorganisms by using high pressure and steam to reach temperatures above 250F (121C), which destroys the cell walls and proteins of the microorganisms, ultimately leading to their death.


Why is it important to use an autoclave in the hydrolysis of protein?

Using an autoclave in the hydrolysis of proteins is important to ensure complete sterilization and to prevent contamination by microorganisms. The high temperature and pressure inside the autoclave also help in breaking down proteins efficiently during hydrolysis. This results in a more controlled and reliable protein hydrolysis process.


What are three leading factors that cause incomplete autoclave sterilization?

Improper loading of the autoclave, such as overcrowding or blocking steam circulation, can lead to incomplete sterilization. Incorrect cycle selection or setting of the autoclave parameters, such as time, temperature, and pressure, may result in inadequate sterilization. Using damaged or expired instruments, incorrect packaging materials, or contaminated items can compromise the sterilization process in the autoclave.


What is the function of autoclave in the laboratory?

An autoclave in the laboratory is used to sterilize equipment and supplies by using high pressure and high temperature steam. This process ensures that any microbial contaminants are destroyed, making the equipment safe for use in experiments and procedures.

Related questions

What are limitation of using autoclave?

Some limitations of using an autoclave include the size restrictions for items that can be sterilized, the need for proper training to operate safely, and the potential for damage to heat-sensitive materials. Additionally, autoclaves require regular maintenance and validation to ensure proper functioning and sterilization efficacy.


What is the meaning of autoclave pound?

It seems there may be a misunderstanding. An autoclave is a machine used for sterilizing equipment or objects using high pressure and steam. There is no standard meaning for "autoclave pound" in this context.


Is it true that there is no limitation in using heat?

No.


Is it safe to autoclave a volumetric flask?

No, it is not safe to autoclave a volumetric flask as the high temperature and pressure in an autoclave can damage the flask's accuracy and calibration markings. It is recommended to clean volumetric flasks using alternative methods such as washing with detergent and disinfecting with ethanol or bleach.


What type of water must be used in a steam autoclave?

Distilled water should be used in a steam autoclave to prevent mineral deposits and prolong the life of the equipment. Using tap water or other types of water can damage the autoclave and compromise the sterilization process.


How much the autoclave volume?

how much autoclave volume ? laboratores


When was Autoclave - album - created?

Autoclave - album - was created in 1991.


Is there any limitation in the length of the query string when using GET or POST method?

There is no limitation for the POST method but for the GET method it is 256 characters


Why is it important to use an autoclave in the hydrolysis of protein?

Using an autoclave in the hydrolysis of proteins is important to ensure complete sterilization and to prevent contamination by microorganisms. The high temperature and pressure inside the autoclave also help in breaking down proteins efficiently during hydrolysis. This results in a more controlled and reliable protein hydrolysis process.


How can I sterilize needles used in tattooing?

You really should not be reusing needles to tattoo someone. If you are going to though, the only way to properly sterilize them is by using an autoclave. An autoclave uses extreme pressure and heat to kill germs and bacteria.


What water do you use in an autoclave?

Demineralised water should best be used in an autoclave.


Which is the best Autoclave company in India for dental clinics?

Thymol India -- Leading Autoclave Machine Manufacturing Company in India. Quality autoclave, fully automatic, portal dental autoclaves available at very reasonable prices.