"TO CONTAIN" VERSUS "TO DELIVER"
Proper use of calibrated volumetric glassware requires that the user be
informed whether the ware is a "to contain" or "to deliver" vessel. All Kimble
calibrated ware is marked either "TC" or "TD". When the graduation line
denotes the volume contained in the calibrated vessel, the ware is marked
"TC". When the graduation line indicates the volume delivered from the vessel,
the ware is marked "TD". When a vessel is calibrated "TD", it differs from a
"TC" calibrated vessel in that a drainage holdback error, the amount of water
required to wet the inner surface of the vessel in contact with the water, is
added to the "TC" volume. The "TD" vessel then delivers the same volume as
contained in a "TC" vessel. Product descriptions in this catalog usually state
the method of calibration used. (The International Standards Organization
designation for "to contain" is "IN" and "to deliver", is "EX".)
From www.kimble-kontes.com
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A pipette that is meant to contain liquid is used for drawing and transferring specific volumes of liquid. A pipette that is meant to deliver liquid is used for dispensing specific volumes of liquid accurately. The main difference lies in their primary functions - one is used for uptake, while the other is used for dispensing.
When air is removed from the siphon by the pipette, the atmospheric pressure pushes the liquid into the siphon to fill the vacuum created. This creates a pressure difference between the two ends of the siphon, causing the liquid to flow into it.
A volumetric pipette is used to accurately measure and transfer a precise volume of liquid. It is designed to deliver a single, specific volume of liquid with high accuracy and precision. Volumetric pipettes are commonly used in analytical chemistry and laboratory settings where precise measurements are crucial.
The volumetric pipette is more accurate in measurement compared to the pipette. Volumetric pipettes have a single calibration mark and are designed to deliver a specific volume of liquid accurately, while standard pipettes have graduations along the stem and are typically less precise.
A pipette controller is a piece of laboratory equipment that makes use of air pressure to accurately dispense liquids from a pipette. By controlling the air pressure within the device, users can efficiently and precisely transfer liquids in scientific experiments.
To measure 30 micro liters using a 100 micro liter pipette that only goes down to 55 micro liters, you would need to pipette 55 micro liters first. Then, dispense the liquid, and pipette 25 micro liters again using the same pipette. This way, you will have measured a total of 30 micro liters.