place a drop oil on the side
place a drop oil on the side
When using the oil immersion objective, the oil has the same refractive index as the glass. So it is like an extension of the lens. Water does not have the same refraction index as glass, so the image would not be as clear.
Cedar wood oil
A high-quality microscope objective lens with a specialized coating is typically used for oil immersion microscopy. The lens is designed to work with a specific type of oil (usually immersion oil) to enhance resolution and numerical aperture by reducing light loss due to refraction. Oil immersion microscopy is commonly used for high-magnification studies requiring precise imaging, such as in medical or biological research.
Before rotating the oil immersion lens into place, make sure that the specimen is in focus using a lower magnification objective. Ensure that the area you wish to observe is centered in the field of view and add a small drop of immersion oil to the slide.
Oil immersion is used to minimize light refraction and increase resolution by eliminating the air gap between the lens and the specimen. The oil has a refractive index close to that of glass, allowing more light to enter the lens and improving the quality of the image.
If you are using the oil immersion objective on a microscope, you must use oil to increase the resolution of the lens. These lens are used at very high magnification.
Heavy mineral oil is a more viscous substance, light mineral oil is less viscous. Therapeutically, Light Mineral Oil is used as an emollient for the skin, while Heavy Mineral Oil is used as a Cathartic/Laxative for the bowel.
immersion oil
mineral oil
The purpose to use oil is to make it clear in the microscope. Oil is the quintessential "tool" to make oil immersion magnification possible. Oil has the same refractive index as glass, therefore the light is not bent by the air that would otherwise be in the oil's place.