PCR is a biotechnological method to amplify your gene (DNA) of your interest. It produce millions of your DNA fragments hence used in cloning. There are variants of this method using the same thermocycling principle such as touch down PCR, gradient PCR, RFLP, multiplex PCR, Q PCR, RT PCR and so on.
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Real time PCR has been around for over twenty five years. It was
revolutionized in the mid 1980's. Real time PCR helps view how much
is increased in a DNA sample.